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Table 4. antigens selected by the ANTIGENome technology.

https://doi.org/10.1371/journal.pone.0064422.t004

Of the 23 candidates selected by the ANTIGENome technology, we evaluated 8 well conserved (see Table 5 ) and readily recombinant expressed antigens that had shown some promise in a preliminary mouse study in more detail for their potential to elicit protective immune response in vivo ( Le Palm Polka Dot Print Bardot Bikini Top Coral polka dot Le Palm Clearance Websites Clearance Genuine Ebay Sale Online Cheap Huge Surprise UxS52yfeDJ
). The rate of M. catarrhalis clearance from mouse lungs in response to immunization with recombinant antigens was assessed using a mouse pulmonary clearance model ( Figure 2 ). Mice were immunized intranasally 3 times at 3 week intervals and challenged intranasally with 40 µL of approximately 5×10 6 live M. catarrhalis RH4 (actual CFU varied between 3.8×10 6 to 5.9×10 6 ) 3 weeks after the last boost. Bacterial CFU were determined in lungs 6 hours post infection and systemic antibody titers after vaccination of mice were determined by ELISA ( Figure 2 ).

Figure 1. Structural features of 8 potential vaccine candidates.

MCR_0076, TonB-dependent receptor; MCR_0196, MltB; lytic murein transglycosylase; MCR_0686, peptide methionine sulfoxide reductase MsrA/MsrB; MCR_0996, hypothetical protein; MCR_1003, LysM domain protein; MCR_1010, D-alanyl-D-alanine carboxypeptidase; MCR_1303, oligopeptide ABC transport system substrate binding protein; MCR_1416, cytochrome c class II, Msp22. SP, signal peptide; LP, signal peptide for lipidation; Plug, an independent folding subunit blocking the pore until the channel is bound by a ligand; PGBD1, peptidoglycan binding-like; MsrA, methionine sulfoxide reductase A; SelR, seleno protein R; LysM, lysine motif; SBP bac 5, bacterial extracellular solute-binding protein family 5. Light grey bars represent the recombinant protein (fragments). Thin black bars delineate epitope containing regions covered by clones selected by the ANTIGENome technology with human IgGs.

https://doi.org/10.1371/journal.pone.0064422.g001

Figure 2. Pulmonary clearance of RH4 after intranasal challenge following intranasal immunization with 8 selected antigens.

Pulmonary clearance 6 hours after intranasal challenge with ∼5×10 6 CFU M. catarrhalis , in mice immunized with purified, IC31 ® adjuvanted recombinant proteins, IC31 ® adjuvant without proteins in PBS, or PBS without adjuvant. The mean values of the combined, normalized results from 2 to 6 independent experiments are shown. Error bars represent the standard error of the mean. (A) Bacterial CFU recovered from all experiments; (B) bacterial CFU recovered from experiments after exclusion of sterile lung cultures. Black bars: negative and positive controls (data from 6 experiments), grey bars: data from 2 to 3 independent experiments in which different antigens were tested. (C) ELISA measuring IgG levels to the respective recombinant proteins in serum from mice immunized intranasally with purified recombinant proteins as noted below the x-axis. For the controls (IC31 ® alone or PBS), IgG levels were determined using a mix of all recombinant proteins. Endpoint titers were expressed as the last dilution that gave an absorbance of at least 0.1 at 405 nm. Median values with the interquartile range from 2 to 6 independent experiments using 10 sera (10 mice per group) per experiment are shown. **, statistically highly significant (P<0.01), *, statistically significant (P<0.05).

Así que no te estreses con la cámara. Te sugiero que primero intentes utilizar lo que tengas en casa antes de ir de compras. Si finalmente optas por comprar una, sugiero que compres una cámara que tenga, al menos, ajustes de exposición y abertura manuales.

¿Qué comprar? Bueno, hay un montón de cámaras fantásticas en el mercado y teniendo en cuenta el presupuesto de un vendedor online, sugiero algo en la línea de una Canon Power Shot SX510, que es más que suficiente para conseguir el trabajo deseado. Esta cámara se vende por menos de 200 € , tiene ajustes manuales, toma imágenes claras y nítidas, y tiene la opción de wifi. Recuerda que también puedes obtener una gran cantidad de cámaras de segunda mano en eBay o en otros portales similares, y aún más asequibles!.

200 € – Canon Powershot SX510

2. Tarjeta de Memoria (10 €)

No te olvides de la tarjeta de memoria SD! Esto es lo que tu cámara va a utilizar para almacenar tus imágenes durante la sesión de fotos. Hoy en día las tarjetas de memoria son baratas y una tarjeta SD de alta capacidad (16 GB) no debería costar más de 10 €.

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3. Trípode (10 €)

Un trípode es una parte esencial en tu estudio casero, ya que te permitirá colocar la cámara y trabajar en entornos en los que la iluminación no es la más óptima (como tu sala de estar!).

En la segunda parte de este artículo, voy a mostrarte cómo ajustar la configuración manual de la cámara de acuerdo con la luz en el estudio. No es necesario gastarse un montón de dinero en un trípode, pero es absolutamente necesario comprar uno. A continuación te indico una buena opción de trípode de bajo presupuesto:

10 € – Trípode para cámara digital Canon, Sony, Nikon

4. Rollo de Papel Blanco

Para crear un buen fondo transparente te recomiendo que compres un gran rollo de papel blanco para poner detrás de tu producto. Puedes comprar rollos de papel blanco en la mayoría de papelerías o tiendas de material de oficina y de cámaras.

Es importante contar con un largo rollo de papel blanco porque será necesario para crear un “fondo continuado” (un fondo transparente detrás del producto) con el fin de reducir el trabajo de post-producción.

El ancho del rollo de papel debe ser mayor que el producto que estás fotografiando. Recomiendo un buen tamaño estándar de 130 cm de ancho. Puedes encontrar otros que sean más pequeños y más anchos, pero esta longitud es universal para casi todo. Creo que el único producto para el que este tamaño no podría ir bien sería para productos como muebles o ropa en modelos. En estos casos, necesitarías algo tan amplio como la pared.

Surf at Center of Portugal

From Figueira da Foz with Europe’s best right to the gigantic waves of Nazaré and the absolutely perfect tubular waves of Peniche, there is a sea of possibilities to surf on.

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The delightful town of Óbidos, with white houses adorned with bougainvilleas and honeysuckle was captured from the Moors by the first king of Portugal, D. Afonso Henriques, in 1148. D. Dinis later presented it to his wife, Queen Santa Isabel. From then until 1883, the town of Óbidos and the surrounding land was always the property of the queens of Portugal.

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They can be very small dots in the world map, but it was thanks to them that the fate of a country was changed. Strategically placed next to the Spanish border, Portugal’s historical villages are the work result of many generations of Kings who were concerned about territorial defence and decided to populate and fortify the area.

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The town of Batalha developed alongside the Monastery of Santa Maria de Vitória, constructed in 1386 to keep a vow by Portuguese King Dom João I to the Virgin Mary that he would build it if Portugal defeated Castile at the Battle of Aljubarrota on 14th August 1385.

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Known as the “Portuguese Venice”, the city is quietly dominated by the Ria de Aveiro, described by Saramago as “a living body that connects the land to the sea like a huge heart.”

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Serra da Estrela Natural Park is an exceptional place. It expands over 101 thousand hectares distributed across six municipalities and it was the first natural park to be created in Portugal, also holding the record for the largest protected area in the country.

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Fátima is one of the most spectacular religious sanctuaries of the world, a place of tranquility and the meeting point of Christians, Catholics, Protestants, Orthodoxs, Muslims, Jews, Hindus, Buddhists and agnostics from the four corners of the world. Discover the history of this enchanting place of confinement and inner peace, as well as its most iconic spots!

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Alcobaça lies in the valleys of the Rivers Alcoa and Baça, which according to some authors is the origin of its name. It has also been suggested that it was the Arabic name of the place which was split to name the two rivers.

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Occupied since the Castro era, the History of Viseu is closely connected with the History of Portugal.

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If “Coimbra is a lesson”, as the popular fado song says, Coimbra is firstly a lesson of Portuguese History.

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Distinct architecture, a Templar castle, 15th century doors and churches, Baroque gardens and manor houses belonging to families with Historical roots. If you visit Castelo Branco, the capital of “Beira Baixa”, you will see all this in a place which combines tradition and novelty.

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Peniche: a surfers’ paradise!

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