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In addition, we also observed some interesting genes and features in the GIs shared between W1481 and some of the F. nucleatum strains. For example, we found that the horizontally transferred GI3, which was shared by W1481 with the strains of subsp. animalis (11_3_2), subsp. fusiforme (ATCC51190) and subsp. nucleatum (ATCC23726), carries the tripartite ATP-independent (TRAP)-like transporter genes, encoding for TRAP-type C4-dicarboxylate transport system-periplasmic component, TRAP-type C4-dicarboxylate transport system-small permease component and TRAP-type C4-dicarboxylate transport system-large permease component. These TRAP transporters are a high-affinity transport system for the C4-dicarboxylates malate, succinate, and fumarate ( Forward et al. 1997 ). Strikingly, the GI4 which was shared by W1481 with the strains of subsp. polymorphum (ATCC10953 F401) and three strains of subsp. animalis (F0419, D11 7_1), contain a cluster of eight putative CRISPRs genes which may provide the host with acquired and heritable resistance to foreign DNA ( Horvath and Barrangou 2010 ; Marraffini and Sontheimer 2010 ; Jore et al. 2012 ). The distribution of the GIs among the strains indicates that W1481 share a number of GIs with the strains of subsp. animalis , vincentii and polymorphum .

To better understand the pathogenic potential of W1481, we performed a comparative pathogenomic analysis of W1481 along with the strains of the five subspecies of F. nucleatum ( fig. 6 ). W1481 showed the presence of a number of virulence genes which were shared by all or most of the other strains under study mostly related to lipopolysaccharide layer of the bacterial cell surface, such as galE (UDP-glucose 4-epimerase), galU (glucose-1-phosphate uridylyltransferase), gtaB (UTP–glucose-1-phosphate uridylyltransferase), acpXL (acyl carrier protein), kdsA (2-dehydro-3-deoxyphosphooctonate aldolase), lpxA (UDP-N-acetylglucosamine acyltransferase), gmhA/lpcA (phosphoheptose isomerase) and eno (phosphopyruvate hydratase). W1481 also shared the presence of groEL a 60-kDa chaperonin, htpB ( Hsp60 , 60K heat shock protein), hemL (glutamate-1-semialdehyde aminotransferase), tuf (elongation factor Tu) and plr/gapA (glyceraldehyde-3-phosphate dehydrogenase) with the other strains.

F ig . 6.—
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Comparative pathogenomic analysis of W1481. The threshold used in this analysis was 50% sequence identity and 50% sequence completeness.

F ig . 6.—
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Comparative pathogenomic analysis of W1481. The threshold used in this analysis was 50% sequence identity and 50% sequence completeness.

One interesting observation was the presence of ddhABC genes which are related to CDP-glucose pathways. These genes were present in W1481 and only one other strain of F. nucleatum subsp. vincentii ATCC49256. ddhABC genes are involved in lipopolysaccharide O-antigen biosynthesis which have been reported to play a crucial role in effective colonization of host tissues, and evasion of the host immune system by gram negative bacteria ( Lerouge and Vanderleyden 2002 ; Skurnik and Bengoechea 2003 ). None of the other strains under study showed the presence of these genes. W1481 also showed the presence of Putative O-antigen ligase WaaL which is responsible for the ligation of O-antigen onto the core region of the lipid A-core block a crucial step in the lipopolysaccharide biosynthetic pathway ( Han et al. 2012 ).

I use an older Sony HD camera which offers 25P, 50i and 50P frame rates. 50P (.MTS) recorded internally and transcoded to Prores 422 25P with Convert v3 (uses PTS) results in excellent 50% slow motion but when I shoot 50i or 25P, I record externally to ProRes HQ 422. 'Non scientific' visual tests that I've conducted comparing the smoothness of the resultant pan, suggests recording 50i then running re-wrap to ProRes 25P with de-interlace checked - same sharp 25P image with smoother motion. You can download a free trial of Convert v3 and experiment: http://hdcinematics.com/Convert-V3.html
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Re: How to avoid motion artifacts at 24p/25p/30p?

Wed Jul 06, 2016 5:11 pm

There is a Time Shaper app it can help http://tessive.com/ It mimic film shutter look. It can work with 60fps source but not too perfect. Needs 120fps or more. There is also a lot of very useful info and research about film vs digital shutter in tech blog http://tessive.com/tech-blog/ p.s. Actually RED acquire that film shutter hardware addon developed by these team.
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Wed Jul 06, 2016 10:06 pm

I must to shoot this weekend a commercial with scene that happening to a lake (in wilde with birds and fishmans) but also some interiors to a building. I thinking to shoot wild scene at 50 fps for slow-motion with birds and fishes and specialy no jerky when i do pan and tilt, but what happend if i must to deliver this project at 25 fps? How i can transform the fluent 50 fps movie in a 25 fps movie without jerky pan and tilt? How i can use in same seqvence a 50 fps movie with a 25 fps movie?

Craig Marshall

Re: How to avoid motion artifacts at 24p/25p/30p?

Wed Jul 06, 2016 10:19 pm

Do you want to create a 25p video from your 50P source with 50% slow motion applied or not? If you are happy to have 50% slow motion, the best and smoothest solution I have found is to apply the Post Time Stamp (PTS) 50P to 25P speed change using Convert v3 (linked above) I prefer to apply these changes to footage before I go into the edit so I have all files correctly timecoded and reel numbered.
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Craig Marshall

Re: How to avoid motion artifacts at 24p/25p/30p?

Wed Jul 06, 2016 10:25 pm

The treating physician for this patient was informed of the unusual nature of this organism that was causing a UTI. This was the first known UTI experienced by the patient, and the physician stated that this patient had no known urinary tract abnormality. 1 , - 3 No urinalysis results were available. The patient had not yet been prescribed antibiotics. We requested a new urine specimen so we could plate to CHOC for a colony count and reisolation of the organism; however, no additional specimen was received. A follow-up call to the physician yielded no response regarding the treatment that the patient had received or any recurrence of the UTI.

The second case involves a 60-year-old African American man with a history of kidney cancer. 4 The urinalysis results showed greater than 182 white blood cells/low power field (WBC/LPF), a positive result for nitrates, and the presence of 1+ protein. The preliminary plate reading showed no growth on BLD, MacConkey's agar (MAC), and cysteine naladixic acid agar (CNA). The plates were returned to the incubator for further incubation.

The second day reading showed pinpoint-sized colonies on BLD, and the plates were reincubated overnight for mature growth ( Image 2 ). The following day, the pinpoint colonies were gram stained and reported as gram negative bacilli or possibly gram variable bacilli; we theorized that these colonies were possibly made up of Gardnerella spp. The colonies were subcultured to CNA for biochemical testing to confirm identification. Subsequently, 2 days passed while waiting for sufficient growth, but the organism failed to grow. The original specimen had been discarded before identification of the organism was made, so reinoculation on CHOC was not possible.

Image 2
Image 2
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Download slide

Hemophilus parainfluenzae on BLD agar plate from 50 year old male.

Image 2
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Download slide

Hemophilus parainfluenzae on BLD agar plate from 50 year old male.

The organism was then subcultured to CHOC and incubated in 5% CO 2 at 35°C overnight. The CHOC grew colonies typical of Haemophilus spp. RapID NH kit testing was performed, and the organism was identified as Haemophilus parainfluenzae . Susceptibility testing was performed. The organism was susceptible to ampicillin, azithromycin, cefaclor, cefuroxime, and ciprofloxacin. It showed intermediate resistance to tetracycline and resistance to trimethoprim sulfamethoxazole. The patient was originally prescribed Bactrim but was switched to ciprofloxacin 500 mg twice daily for 20 days. At the next follow-up appointment, the infection had been resolved.

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¿Nunca te has preguntado cuántos smartphones se venden al cabo del año en el mundo ? Posiblemente sí. Hacer una estimación certera es complicada dado que tendríamos que tener las cifras de ventas de todas las marcas del planeta –solo en Android hay 1.300–, y las firmas prefieren guardarse estos datos para ellas y sus inversores. Sin embargo, las consultoras hacen bien su trabajo y llevan a cabo grandes labores de investigación y análisis para ofrecernos estas cifras, y hoy, en pleno ecuador de 2018, por fin sabemos cuántos móviles Android –y iPhone– se vendieron en 2017.

Han sido los analistas de Garnet los que han publicado el informe con estos datos y, como te podrás imaginar, el sistema operativo de Google sale muy, muy, muy bien parado. De acuerdo a Gartner, Android consiguió un 85,9% de cuota de mercado –algo que le ha valido a la Gran G una buena multa –, mientras que Apple y su iOS se quedaron con un modesto 14% . El 0,1% restante se lo quedan otros sistemas operativos minoritarios.

En términos absolutos, solo en 2017 se vendieron 1.300 millones de smartphones Android , una cifra muy lejana de los 215 millones de iPhone y los 1,5 millones que representan los demás sistemas operativos. El motivo de esta abismal diferencia es evidente: Android es un sistema abierto que cualquier empresa puede usar para lanzar sus terminales, mientras que iOS está diseñado por y para los iPhone (y demás iDevices), nada más.

Por otro lado, y si bien es cierto que Android aplasta cruelmente las cifras de ventas de iOS, los “taliandroid” tenemos que ponernos un punto en la boca cuando llega el turno de hablar de fragmentación . Mientras que iOS 11, la última versión estable del sistema operativo de Apple, está instalada en casi un 70% de los iPhone, en Android, bueno, se podría decir que la situación es un poco distinta.

Este es un problema con el que Google tiene que lidiar . La fragmentación en Android, a pesar de ser un sistema operativo mucho más extendido y popular, sigue siendo un desastre. No es normal que haya casi tantos usuarios en Android Oreo como en Lollipop. ¿Conseguirá Project Treble arreglar este tema? Solo el tiempo lo dirá, aunque lo que hemos visto hasta el momento no parece demasiado alentador.

Fuente : Gartner, vía GadgetsNow

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Para que te hagas una idea, 1.300 millones de smartphones Android equivalen a 28 smartphones por cada ciudadano español.

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